α-Synuclein-Luciferase Bioluminescent Stable H4 Cell Line (H4 S1S2)

Unit

1×10⁶ cells / 1.0 ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Brain

Organism

Human (H. sapiens)

Donor History

Male

White

Neuorglioma

Growth Properties

Adherent

fibroblast-like with large cytoplasm; elongated and spindly when too confluent

Growth Conditions

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for cell adhesion to the culture vessels. PriGrow C Medium (TM100) + 10% FBS + 200ug/ml Hygromycin B (G265) + 200ug/ml G418 (G262) + 1 ug/ml tetracycline* + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. *Expression of the construct is tetracycline regulated – TET-OFF***Cell growth and proliferation is better when seeded at higher densities; do not seed too sparsely**

Immortalization Method

Full Information

https://www.abmgood.com/α-Synuclein-Luciferase-Bioluminescent-Stable-H4-Cell-Line-H4-S1S2-.html

This H4 neuroglioma cell line stably expresses bioluminescent-protein complementation pairs of alpha-synuclein (α-synuclein) and luciferase (synLuc1/synLuc2) under tetracycline regulatable expression (tet-off). α-synuclein is implicated in Lewy body related disorders like Parkinsons, dementia, Lewy bodies, and multiple system atrophy. Therefore, this cell line is suitable for studies investigating the pathogenic role of α-synuclein in such diseases. Complementation of α-synuclein with luciferase allows for in vitro imaging to investigate protein misfolding and aggregation. Control cell line:Cat. T3427 – Human Wild-Type Alpha-Synuclein H4 Cell LineCat. T3428 – Full-length Gaussia Luciferase Expressing H4 Stable Cell Line (HgLuc) Complete list of cell lines from this panel:Cat. T3429 – α-Synuclein-Luciferase Bioluminescent Stable H4 Cell Line (H4 S1S2)Cat. T3430 – α-Synuclein-Venus Fluorescent Stable H4 Cell Line (H4 V1S/SV2)Cat. T3441 – Full-length Venus Expressing H4 Stable Cell Line (HgLuc) Cat. T3458 – Mutant Alpha-Synuclein (Ala53Thr) Stable H4 Cell LineCat. T3459 – Mutant Alpha-Synuclein (Ala30Pro) Stable H4 Cell Line

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Unit	1×10⁶ cells / 1.0 ml
Cell Type	Stable (KO/Expressing) Cells
Tissue Type	Brain
Organism	Human (H. sapiens)
Donor History	Male , White , 37 , Neuorglioma
Growth Properties	Adherent , fibroblast-like with large cytoplasm; elongated and spindly when too confluent
Growth Conditions	PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for cell adhesion to the culture vessels. PriGrow C Medium (TM100) + 10% FBS + 200ug/ml Hygromycin B (G265) + 200ug/ml G418 (G262) + 1 ug/ml tetracycline* + 1% Penicillin/Streptomycin Solution (G255) , 37.0°C , 5% CO₂. Expression of the construct is tetracycline regulated – TET-OFFCell growth and proliferation is better when seeded at higher densities; do not seed too sparsely
Immortalization Method
Full Information	https://www.abmgood.com/α-Synuclein-Luciferase-Bioluminescent-Stable-H4-Cell-Line-H4-S1S2-.html

Přidat mezi Oblíbené

Katalogové číslo: T3429 Kategorie: Buněčné linie, Stable (KO/Expression) Cell Lines

Popis
O značce

Popis

O značce

Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.

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