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γ-secretase Deficient E-Cre-ERT2 Stable Expressing Rat1 (AD6 Clone 1) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Rat (R. norvegicus) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 mg/ml Hygromycin (G265) ,10 µg/ml Blasticidin ,4 mg/ml Geneticin/G418 (G271) and 10-6 M tamoxifen for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
This rat cell line expresses a chimeric molecule containing a delta E-like fragment of Notch1 fused to a vesicular stomatitis virus epitope the Cre recombinase and a modified hormone binding domain of the estrogen receptor. The delivery of Cre recombinase depends on any event including gamma-secretase processing that releases the intracellular domain of Notch1 from the membrane. Nuclear activity of the Cre-ER-T2 chimera depends on the presence of tamoxifen. This cell line was derived from clones deficient in gamma-secretase activity thus displaying impaired activity on Notch. Reporter construct for the recombinase activity consists of an antibiotic resistance gene (hygromycin). This cell line would be well-suited for research regarding the functions of the Notch receptor gamma-secretase enzyme activity and their involvement in cellular signalling.
