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ADRM1 Stable Knockout HCT116 (Clone B11) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,48 ,Caucasian ,Colorectal carcinoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.4 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/adrm1-stable-knockout-hct116-clone-b11-cell-line.html |
ADRM1 or Adhesion regulating molecule 1, is a component of the proteosome and ubiquitin receptor that recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Known to undergo O-linked glycosylation, the dysregulation of ADRM1 has been implicated in carcinogenesis making this protein a possible target for drug studies. ADRM1 stable knockout HCT116 (Clone B11) cell line was generated through CRISPR-Cas 9 directed knock exon 4 within the ADRM1 sequence. Exon 4 was replaced with a puromycin resistance cassette and pBluescriptII, allowing for the selection of successfully transformed cells via puromycin resistance. Control cells available (T8240).
