AN3 Ca Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Reproductive system cancer cell lines

Organism

Human

Donor History

Adenocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: EMEM (MEM Eagle)

,

w: 2 mM L-Glutamine

,

w: 2.2 g/L NaHCO3

,

w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Seeding density: An initial seeding density of 3 to 4 x 10⁴ cells/cm² is recommended. Later on

,

2 x 10⁴ cells/ cm² will yield a confluent layer in 4 to 5 days. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/AN3-Ca-Cells/300119

SKU: 300119 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The An3 Ca cell line is derived from a human endometrial adenocarcinoma, a type of cancer originating from the lining of the uterus. This cell line is estrogen receptor negative (ER-) and exhibits aggressive tumorigenic potential when assessed in vivo. An3 Ca cells are used extensively in research focused on understanding the molecular and cellular mechanisms underlying endometrial cancer progression, including studies on cancer cell proliferation, metastasis, and response to therapeutic agents.

Characteristically, An3 Ca cells display an epithelial morphology and have been utilized to study the impact of various genetic and environmental factors on cancer cell behavior. Research using this cell line has contributed to identifying potential therapeutic targets and understanding the resistance mechanisms against conventional treatments. They serve as a valuable model for evaluating new drugs or treatment strategies that could be effective against aggressive forms of endometrial cancer.

Overall, the An3 Ca cell line is instrumental in advancing the scientific knowledge of endometrial adenocarcinoma, offering insights that might lead to more effective interventions for this challenging and often lethal disease.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.