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ARL13-B Stably Expressing HEK 293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.8 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/arl13-b-stably-expressing-hek-293-cell-line.html |
ARL13-B is a small GTPase belonging to the class of Arf/Arl family within the Ras superfamily of small GTPases involved in diverse cellular functions. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs and mutations in this gene leads to the classical form of Joubert Syndrome. Since ARL-13B is linked to a wide range of ciliary processes, this cell line could be helpful to study various processess related to cilium formation, function and transport.
