B-CPAP Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Head-Neck cancer cell lines |
| Organism |
Human |
| Donor History |
Thyroid carcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
B-CPAP is a human papillary thyroid carcinoma cell line that was established from the primary tumor of a 74-year-old woman. The cell line exhibits epithelial-like morphology and is commonly used in research to study thyroid cancer biology, including mechanisms of tumorigenesis and metastasis. B-CPAP cells are notable for harboring a BRAF V600E mutation, which is a common genetic alteration associated with aggressive thyroid cancers and serves as a critical model for evaluating BRAF inhibitors as therapeutic agents.
In addition to the BRAF mutation, B-CPAP cells express thyroid-specific markers such as thyroglobulin and thyroid-stimulating hormone receptor, making them a valuable model for studying thyroid gland function and pathology. They have been extensively used in studies investigating the signaling pathways involved in thyroid cancer progression, including MAPK/ERK pathway activation. These cells are also employed in drug resistance and apoptosis studies, providing insights into the mechanisms that might underpin therapeutic failures in thyroid cancer treatments.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

