B-LCL-HROC195 Cells

B-LCL-HROC195 is an Epstein-Barr virus (EBV)-immortalized human B lymphoblastoid cell line established from B lymphocytes isolated from either tumor tissue or peripheral blood of an adult patient. The cells were generated by ex vivo infection with EBV-containing supernatant derived from the B95/8 marmoset cell line in the presence of cyclosporin A to suppress T- and NK-cell outgrowth. Following several weeks of culture, stable lymphoblastoid outgrowth was achieved, resulting in a continuously proliferating monoclonal or oligoclonal B-cell population suitable for long-term in vitro expansion.

Immunophenotypically, B-LCL-HROC195 exhibits a mature and activated B-cell profile characterized by expression of CD19 and CD20, along with high levels of activation and maturation markers such as CD23 and CD80. Strong expression of MHC class I and class II molecules indicates preserved antigen-presenting capacity. Depending on the individual clone, variable expression of differentiation-associated markers such as CD27, CD38, or CD138 may be observed, reflecting different stages of B-cell maturation. The cells are negative for T-cell markers, confirming lineage specificity.

Functionally, B-LCL-HROC195 secretes immunoglobulin of a defined isotype (e.g., IgG, IgM, or IgA), which remains stable during prolonged culture. The secreted antibodies can be collected from culture supernatants and used for downstream applications, including antigen-binding assays, tumor cell recognition studies, or identification of disease-associated antigens. As an EBV-immortalized B-cell model, B-LCL-HROC195 provides a robust in vitro platform for investigating humoral immune responses, B-cell activation and differentiation, and antibody-mediated mechanisms in the context of tumor immunology or systemic immune responses.