c-Kit Y703F/Y936F Stable Ba/F3 Cell Line

c-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system and has nine key tyrosine residues. Y703 and Y936 residues of the c-Kit receptor are involved in recruiting signaling molecules and are ligand-induced phosphorylation sites for Grb2. c-Kit Y703F/Y936F-expressing Ba/F3 cells (with GNNK- isoform) has a Y-to-F mutation at position 703 and 936, which results in reduced binding for Grb2. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. Together with the wild-type c-Kit stable Ba/F3 cells (Cat. No. T3041 and T3042), these cells may be utilized in studies involving effects of tyrosine residues on signaling pathways.