C6-Luc Cells

C6-Luc is a genetically modified derivative of the rat C6 glioma cell line, which was originally established from a N-nitrosomethylurea-induced glial brain tumour in a Wistar rat by Benda et al. (1968). The parental C6 line was stably transfected with a eukaryotic expression plasmid encoding luciferase under control of a constitutive promoter, followed by selection with hygromycin B to obtain stably expressing monoclonal populations.C6-Luc cells constitutively express luciferase and emit bioluminescent signal in the presence of a luciferin substrate, enabling real-time, non-invasive monitoring of tumour growth by bioluminescence imaging (BLI) in live animals. The line is used to establish orthotopic syngeneic glioma models in Wistar or Sprague-Dawley rats following intracranial stereotactic implantation, and in subcutaneous xenograft models. Applications include evaluation of anti-glioma therapies, drug penetration across the blood-brain barrier, and development of brain tumour imaging platforms.