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Biochemie
Imunoanalýza
CBFβ Stable Knockout HepG2 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,15 ,White ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/cbfb-stable-knockout-hepg2-cell-line.html |
CBFbeta is the non-DNA-binding subunit of the core binding factor (CBF) transcription factor. Derived from HepG2 cells; CBFbeta was knocked out using CRISPR/Cas9. This cell line was developed for research regarding the role of CBFbeta in Hepatitis B virus replication.
