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Biochemie
Imunoanalýza
CEA-2 Stable MC-38 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57BL6 mouse ,Colon adenocarcinoma cells |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 5% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 250 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
MC38 murine colonic adenocarcinoma cells were transduced with a retrovirus containing the human Carcinoembryonic antigen (CEA) gene. The CEA gene expressed in T6408 is a single M: 70000 glycosylated immunoreactive product. The CEA gene present in T6408 was partially sequenced and found to have a deletion of two of the three repeated domains contained in CEA. Despite the loss of two domains the CEA product expresses epitopes recognized by multiple anti-CEA MAbs This cell line can be used as a model to study reagents against a carcinoma expressing human CEA.
