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CHCHD5 CRISPR Knockout A549 Cell Line – T1-U23
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.2 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/CHCHD5-CRISPR-Knock-Out-A549-Cell-Line.html |
This cell line (Cat. T3422) features a stable knockout of Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 5 (CHCHD5) protein. CHCHD5 (coiled-coil-helix-coiled-coil-helix domain-containing protein 5) is involved in mitochondrial function and cellular oxidative stress responses. Knocking out this gene in A549 cells can help researchers study the role of CHCHD5 in cancer cell metabolism, resistance to oxidative stress, and potential impact on tumor growth and response to therapies. This cell line provides a valuable tool for dissecting molecular mechanisms of lung cancer and exploring new therapeutic targets related to mitochondrial function and oxidative stress.
