CHO-BCMA Cells

Unit

3 x 10^6 cells/ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Transformed cell lines

Organism

Chinese hamster

Donor History

Chinese hamster ovary

,

non-neoplastic; genetically engineered for BCMA (CD269/TNFRSF17) surface expression

Growth Properties

Adherent/suspension

Growth Conditions

Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1)

,

w: 3.1 g/L Glucose

,

w: 2.5 mM L-Glutamine

,

w: 15 mM HEPES

,

w: 0.5 mM Sodium pyruvate

,

w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 4 x 10⁵ cells/mL. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/CHO-BCMA-Cells/305972

Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.

CHO-BCMA is a stable recombinant Chinese hamster ovary (CHO) cell line engineered to express full-length human B-cell maturation antigen (BCMA; TNFRSF17/CD269). The cell line was generated using a site-specific landing pad recombination system that enables reproducible genomic integration and stable target expression.

BCMA is a transmembrane receptor predominantly expressed on mature B cells and plasma cells, where it regulates cell survival and proliferation through interaction with the ligands BAFF and APRIL. The receptor is highly relevant in multiple myeloma and other B-cell malignancies, making CHO-BCMA cells useful for therapeutic antibody screening, CAR-T and bispecific T-cell engager development, receptor binding studies, and flow cytometry-based assay development.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.