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CHO-CD36 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for CD36 surface expression |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
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CHO-CD36 cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human CD36, a multifunctional class B scavenger receptor also known as platelet glycoprotein IV (GPIV) or fatty acid translocase (FAT). CD36 is broadly involved in lipid uptake, fatty acid metabolism, angiogenesis, inflammation, innate immunity, and cell adhesion. The receptor interacts with a wide range of ligands, including oxidized low-density lipoproteins (oxLDL), long-chain fatty acids, thrombospondin-1, phospholipids, and apoptotic cells. Dysregulated CD36 expression has been implicated in metabolic disorders, atherosclerosis, chronic inflammation, and tumor progression, making recombinant CD36-expressing cell models valuable tools for mechanistic and therapeutic research.
CHO-CD36 cells are widely used for studying receptor-ligand interactions, lipid transport mechanisms, and therapeutic targeting of CD36-associated pathways. These cells support quantitative analysis of ligand binding, receptor internalization, fatty acid uptake, and downstream signaling events linked to oxidative stress, immune modulation, and metabolic adaptation. In oncology research, CHO-CD36 models are useful for investigating the role of CD36 in metastasis, tumor lipid metabolism, and resistance to metabolic stress. The cells are also applied in development and characterization of monoclonal antibodies, small-molecule inhibitors, lipid-targeting therapeutics, and imaging agents directed against CD36. Flow cytometry assays, uptake assays, and high-throughput screening platforms commonly utilize CHO-CD36 cells because of their stable and controlled recombinant receptor expression.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
