Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
CHO-CXCR7 Cells (low)
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for CXCR7 (ACKR3) surface expression (low expression level) |
| Growth Properties |
Monolayer ,adherent |
| Growth Conditions |
Culture medium: Ham's F12 ,w: 1.0 mM stable Glutamine ,w: 1.0 mM Sodium pyruvate ,w: 1.1 g/L NaHCO3 (Cytion article number 820600a). Supplements: Supplement the medium with 10% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
The CHO-CXCR7-Low cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line expressing the CXCR7 receptor at a low level. This cell line was generated using an innovative landing pad technology, allowing for reproducible and precise integration of the CXCR7 gene at a validated genomic locus. CXCR7, also known as ACKR3, is an atypical chemokine receptor involved in various biological processes, including immune modulation and tumor biology. Unlike classical GPCRs, CXCR7 does not signal through G proteins but instead modulates cellular responses by scavenging chemokines, such as CXCL12 and CXCL11, and interacting with CXCR4 through heterodimer formation.
Overexpression of CXCR7 has been implicated in several cancers, including breast, lung, and prostate, where it is associated with enhanced tumor progression, metastasis, and poor prognosis. CXCR7 contributes to cancer progression by altering the tumor microenvironment, promoting angiogenesis, and facilitating cancer cell migration and invasion. Due to its significant role in cancer biology, CXCR7 is an important target in oncology research. The expression of CXCR7 in this cell line was confirmed using flow cytometry.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
