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CHO-EPCAM Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for EpCAM (CD326) surface expression |
| Growth Properties |
Adherent/suspension |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
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CHO-EPCAM cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human epithelial cell adhesion molecule (EpCAM; CD326/TACSTD1), a transmembrane glycoprotein broadly expressed on epithelial tissues and highly upregulated in many epithelial-derived cancers. EpCAM is involved in cell-cell adhesion, proliferation, differentiation, and signaling pathways associated with tumor progression and metastasis. Stable CHO-EPCAM models are commonly generated to provide controlled and reproducible EpCAM surface expression for use in binding, targeting, and functional assays involving therapeutic antibodies and engineered immune cell therapies.
CHO-EPCAM cells are widely used in oncology and translational research for the development and characterization of anti-EpCAM monoclonal antibodies, antibody-drug conjugates, bispecific T-cell engagers, and CAR-T or CAR-NK cell therapies. The model is particularly useful for evaluating antigen-specific binding affinity, receptor occupancy, internalization kinetics, cytotoxicity, and immune synapse formation. In addition, these cells support flow cytometry assay development, high-throughput screening, and validation of EpCAM-targeted imaging agents or diagnostic platforms. Because CHO cells exhibit stable growth characteristics and minimal endogenous expression of many human epithelial markers, they provide a consistent background for recombinant antigen expression studies.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
