Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
CHO-FCGR2B Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for FcγRIIB (CD32B/FCGR2B) surface expression |
| Growth Properties |
Adherent/suspension |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
CHO-FCGR2B cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human Fc gamma receptor IIB (FcγRIIB; FCGR2B/CD32B), a low-affinity inhibitory receptor for the Fc region of immunoglobulin G (IgG). FcγRIIB is broadly expressed on B cells, dendritic cells, monocytes, macrophages, and other immune cell populations, where it functions as a critical negative regulator of immune activation. Upon co-engagement with activating receptors, FcγRIIB recruits phosphatases through its immunoreceptor tyrosine-based inhibitory motif (ITIM), thereby suppressing downstream signaling pathways involved in antibody-mediated immune responses. Dysregulation of FcγRIIB signaling has been implicated in autoimmune disease, chronic inflammation, and altered responses to antibody therapeutics.
CHO-FCGR2B cells are widely used in therapeutic antibody development and immunology research to evaluate Fc-mediated interactions, receptor selectivity, and inhibitory signaling mechanisms. These cells support quantitative assessment of IgG subclass binding, Fc engineering strategies, immune complex interactions, and antibody-dependent modulation of Fcγ receptor pathways. They are particularly valuable for screening monoclonal antibodies, bispecific antibodies, Fc-fusion proteins, and glycoengineered biologics designed to alter FcγRIIB engagement. CHO-FCGR2B models are also frequently applied in flow cytometry assays, receptor occupancy studies, reporter assays, and high-throughput screening platforms intended to characterize Fc receptor specificity and functional activity.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
