CHO-HER2 Cells (medium-high)

Unit

3 x 10^6 cells/ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Hamster cell lines

Organism

Chinese hamster

Donor History

Chinese hamster ovary

,

non-neoplastic; genetically engineered for HER2 (ErbB2/CD340) surface expression (medium-high expression level)

Growth Properties

Monolayer

,

adherent

Growth Conditions

Culture medium: Ham's F12

,

w: 1.0 mM stable Glutamine

,

w: 1.0 mM Sodium pyruvate

,

w: 1.1 g/L NaHCO3 (Cytion article number 820600a). Supplements: Supplement the medium with 10% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/CHO-HER2-Cells-medium-high/305413MH

Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
The CHO-HER2-Medium-high cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line that expresses the HER2 receptor at a medium-high level, approximately 13,000 molecules per cell. This cell line was developed using an innovative landing pad technology, ensuring the HER2 gene’s targeted integration at a specific, pre-validated genomic locus. HER2, also known as ERBB2 or CD340, is a member of the epidermal growth factor receptor (EGFR) family and plays a critical role in regulating cell growth and differentiation. It is a significant biomarker and target in breast and ovarian cancers, where its overexpression is associated with increased tumor aggressiveness and poorer patient outcomes. The expression of CXCR7 in this cell line was confirmed using flow cytometry.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.