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CHO-PDCD1 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for PD-1 (PDCD1/CD279) surface expression |
| Growth Properties |
Adherent/suspension |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
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CHO-PDCD1 cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human programmed cell death protein 1 (PD-1; PDCD1/CD279), an inhibitory immune checkpoint receptor primarily found on activated T cells, B cells, and other immune cell subsets. PD-1 is a type I transmembrane protein belonging to the immunoglobulin superfamily and functions as a critical regulator of immune tolerance through interaction with its ligands PD-L1 (CD274) and PD-L2 (PDCD1LG2). Stable PDCD1-expressing CHO models are commonly developed to provide controlled and reproducible receptor expression for cell-based binding and functional assays.
CHO-PDCD1 cells are widely used in immuno-oncology and therapeutic antibody development workflows, particularly for characterization of checkpoint inhibitor antibodies, ligand-receptor interaction studies, affinity measurements, and flow cytometry-based screening assays. These cells are also suitable for evaluating bispecific antibodies, engineered ligands, CAR-T targeting strategies, and receptor occupancy assays involving the PD-1/PD-L1 signaling axis. Because CHO cells exhibit robust growth characteristics, high transfection efficiency, and low endogenous expression of many human immune receptors, they provide a well-defined background for studying recombinant PD-1 biology and therapeutic targeting.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
