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CHO-STEAP1 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for STEAP1 surface expression |
| Growth Properties |
Adherent/suspension |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
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CHO-STEAP1 cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human six-transmembrane epithelial antigen of the prostate 1 (STEAP1), a cell surface protein highly associated with multiple solid tumors. STEAP1 is a member of the STEAP family of metalloreductases and is characterized by six transmembrane domains with localization predominantly at the plasma membrane and intracellular vesicular compartments. Although its precise physiological function remains incompletely understood, STEAP1 has been implicated in intercellular communication, metal ion homeostasis, redox regulation, and tumor cell proliferation. Elevated STEAP1 expression has been reported in prostate cancer, Ewing sarcoma, bladder cancer, lung cancer, and several other malignancies, making it an important target in oncology-focused therapeutic development.
CHO-STEAP1 cells are widely used for development and characterization of STEAP1-targeted therapeutics, including monoclonal antibodies, antibody-drug conjugates, bispecific T-cell engagers, radioligand therapies, and engineered immune cell approaches such as CAR-T and CAR-NK therapies. The stable recombinant expression system enables quantitative analysis of antibody binding affinity, receptor occupancy, antigen density, internalization behavior, and target-specific cytotoxicity. These cells are also valuable for flow cytometry assay development, epitope mapping, high-throughput screening, and validation of STEAP1-targeted imaging agents. Because CHO cells provide a robust and relatively low-background platform for recombinant protein expression, CHO-STEAP1 models are frequently used for standardized assay development and preclinical therapeutic evaluation.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
