CHO-TACD2 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Hamster cell lines

Organism

Chinese hamster

Donor History

Chinese hamster ovary

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non-neoplastic; genetically engineered for TACD2/TROP2 (GA733-1) surface expression at medium-high level

Growth Properties

Adherent/suspension

Growth Conditions

Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1)

,

w: 3.1 g/L Glucose

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w: 2.5 mM L-Glutamine

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w: 15 mM HEPES

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w: 0.5 mM Sodium pyruvate

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w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

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we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

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or CM-1 (Cytion catalog number 800100)

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which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

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5% CO2

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humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/CHO-TACD2-Cells/305415

Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entities the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please contact us.The CHO-TACD2 cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line engineered to express the TACD2 receptor at a medium-high level, approximately 12,600 molecules per cell. This cell line was developed using an innovative landing pad technology, ensuring precise and reproducible integration of the TACD2 gene at a specific, pre-validated genomic locus. TACD2, also known as TROP2 or GA733-1, is a tumor-associated calcium signal transducer. It plays a critical role in intracellular calcium signaling, which is crucial for various cellular processes, including growth, division, and differentiation. Overexpression of TACD2 has been observed in various carcinomas, such as colorectal, gastric, and pancreatic cancers, making it a potential target for antibody-drug conjugates and immunotherapy.The expression of TACD2 (TROP2) in this cell line was confirmed using flow cytometry.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.