Colo-94H Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Intestine cancer cell lines |
| Organism |
Human |
| Donor History |
Adenocarcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The COLO-94H cell line is a human colorectal adenocarcinoma cell line derived from a metastatic site in an adult patient. These cells are epithelial in nature and exhibit characteristics typical of colorectal cancer, making them valuable for studies focused on cancer biology, drug development, and metastatic mechanisms. COLO-94H cells grow adherently and form a monolayer, which is typical for epithelial cells in culture. They possess a high degree of genetic and phenotypic stability, allowing for reproducible results in various experimental setups.
Researchers utilize the COLO-94H cell line to investigate the molecular and cellular pathways involved in colorectal cancer progression and metastasis. This includes studying the effects of oncogenes, tumor suppressor genes, and signaling pathways such as Wnt, Notch, and PI3K/AKT. Additionally, COLO-94H cells are used to evaluate the efficacy and toxicity of new chemotherapeutic agents and targeted therapies, providing a reliable in vitro model for preclinical testing. Their metastatic origin also makes them suitable for research into the mechanisms of cancer cell dissemination and colonization of secondary sites.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

