Genetika člověka
Mikrobiologické aplikace
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Biochemie
Imunoanalýza
Control shRNA Stable RTPC Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Rat (R. norvegicus) |
| Donor History | |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. DMEM/F-12 (1:1) Medium (TM510) + 10% FBS + 2 mM L-Glutamine (G275) + 10 ng/ml Human EGF (Z100139) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/control-shrna-stable-rtpc-cell-line.html |
RTPC cells are renal proximal tubular cells isolated from the kidney of a rat. Cell line was created by lentiviral transduction of RTPCs using a shRNA control vector (Sigma SHC001); generated as a control for MTP18 shRNA Stable RTPC Cell Line (T6432).
