Genetika člověka
Mikrobiologické aplikace
NGS
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Jednokanálové
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Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
COS-7-HLA-C*05:02 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Monkey (Primate) |
| Donor History |
Cercopithecus aethiops |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
COS-7 cells expressing HLA-C05:02 antigen are crucial for presenting intracellular antigens to cytotoxic T-cells. This stable cell line was created using retroviral transduction and validated via flow cytometry. It is maintained without antibiotics, making it ideal for research on HLA class I molecule interactions, immune responses, and developing therapies for diseases involving HLA-C05:02.
