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Biochemie
Imunoanalýza
DT40 Cells
| Unit |
5 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Avian cell lines |
| Organism |
Chicken |
| Donor History |
Chicken bursal lymphoma |
| Growth Properties |
Suspension |
| Growth Conditions |
Culture medium: DMEM ,w: 4.5 g/L Glucose ,w: 4 mM L-Glutamine ,w: 3.7 g/L NaHCO3 ,w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS ,0.05 mM 2-mercaptoethanol ,5% Chicken serum ,10% Tryptose Phosphate Broth → 2-mercaptoethanol only 2-3 days stable in medium and 24 hrs at 37°C – protect from light. Seeding density: 2 to 4 x 10⁵ cells/ml. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The DT40 cell line is derived from chicken (Gallus gallus) and is primarily used as a model for studying B-cell biology and immunology. Originating from an avian leukosis virus-induced bursal lymphoma, DT40 cells are unique due to their high rate of homologous recombination, making them particularly valuable for gene targeting studies. This characteristic allows for efficient and precise genetic modifications, facilitating research in various fields including DNA repair, chromosomal dynamics, and gene function.
DT40 cells exhibit rapid growth and a stable karyotype, which are advantageous traits for experimental reproducibility and consistency. They are extensively used to study the mechanisms of immunoglobulin gene conversion and diversification, contributing significantly to our understanding of the adaptive immune system. Moreover, DT40 cells are employed in pharmacological research to screen for potential therapeutic compounds and to analyze cellular responses to DNA damage.
In addition to their applications in immunology and genetic research, DT40 cells are also instrumental in investigating cellular signaling pathways. They provide a robust system for dissecting the roles of various proteins and genes in signal transduction, apoptosis, and cell cycle regulation. The ease of genetic manipulation in DT40 cells, combined with their biological relevance, continues to make them a crucial tool in biomedical research. However, it is essential to note that findings in DT40 cells should be validated in mammalian systems due to species-specific differences.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
