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FER Stable Ba/F3 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone Marrow |
| Organism |
Mouse (M. musculus) |
| Donor History |
C3H ,Pro B cells |
| Growth Properties |
Suspension ,round |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 5 ng/ml Mouse IL-3 (Z200145) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
Fusing a kinase domain to a homodimerization domain such as the PNT domain of ETV6 can result in activated kinase. The FER Stable Ba/F3 Cell Line expresses activated human FER tyrosine kinase that is linked to a GFP reporter (SSBP2-FER in MSCV-GFP). Ba/F3 cells expressing activated tyrosine kinase become completely dependent on the kinase activity for survival.
