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Imunoanalýza
GAB1 Stable Knockout Human 8505c Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Thyroid |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,78 |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.8 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/gab1-stable-knockout-human-8505c-cell-line.html |
GAB1 Stable Knockout Human 8505c Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the GAB1 gene (NM_002039) using the following guide RNA sequence: CCTGGCAGCTCTTTACAAGC. GAB1 gene has important implications for B cell malignancies and this cell line is useful in the study of branching tubulogenesis. This cell line is sequenced verified to be biallelic knockout.
