Genetika člověka
Mikrobiologické aplikace
NGS
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Intolerance u zvířat
Biochemie
Imunoanalýza
GALK2+GALE Stable Knockout HEK293T Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/galk2-gale-stable-knockout-hek293t-cell-line.html |
The UDP-galactose-4 epimerase (encoded by GALE) and Galactokinase 2 (GALK2) are both major enzymes in the Leloir pathway, the primary pathway for galactose metabolism. UDP-galactose-4 epimerase modifies galactose and acetyl galactose, generating precursors for O-linked glycolsylation. Deficiencies in these genes can lead to Galactosemia: a condition that is caused by the accumulation of galactose in the blood and tissue of individuals that cannot properly metabolize the galactose in their diets. As this cell line is derived from a human source, it may be useful in the study of glycosylation and how it is affected during instances of disease.
