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Imunoanalýza
Gnpat Stable Mouse Myoblast (161) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Skeletal Muscle |
| Organism |
Mouse (M. musculus) |
| Donor History |
Cross-breed pups of Gnpat Knockout mice (Gnpatᵗᵐ¹ᴶᵘˢᵗ) crossed with SV40 carrying Tg(H2-K1-tsA58)11Kio mice |
| Growth Properties |
Adherent ,radial branching |
| Growth Conditions |
Use of Gelatin Coating Solution (0.1%) (TM063) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 10% horse serum + 0.5% (vol/vol) chick embryo extract + 20 ng/ml recombinant mouse IFN-γ (Z200085) + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂ |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/gnpat-stable-mouse-myoblast-161-cell-line.html |
Cells were isolated from cross-breed pups of Gnpat wildtype mice (Gnpat⁺ᐟ⁺) crossed with SV40 carrying Tg(H2-K1-tsA58)11Kio mice to obtain the Gnpat Stable Mouse Myoblast (161) Cell Line. This cell line is a Gnpat wildtype which may be used alongside with Gnpat knockout cells for study (available at abm; Cat. No. T6278 or T6279).
