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GPX4 Stable Overexpressing HT-1080 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Connective Tissue |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,35 ,Caucasian ,Fibrosarcoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.5 mg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/gpx4-stable-overexpressing-ht-1080-cell-line.html |
Cell line was generated using retrovirus harboring the GFP-GPX4-cyto expression vector constructed with the GFP cDNA fused at the N-terminal of GPX4-cyto cDNA.
