HEK293FT Cells

The HEK293FT cell line is a derivative of the HEK293 cell line, originally derived from human embryonic kidney cells. The “FT” designation indicates that these cells have been transfected with the SV40 large T-antigen gene, which enhances their ability to replicate plasmid vectors containing the SV40 origin of replication. This modification makes 293FT cells particularly useful for high-efficiency production of viral vectors, such as lentiviruses and adenoviruses, and for transfection studies in molecular biology and gene therapy research.
HEK293FT cells exhibit an epithelial morphology and grow rapidly in culture, providing a robust and reliable system for producing high-titer viral stocks. They retain many of the characteristics of the parental HEK293 cells, including high transfection efficiency and the ability to support the replication of recombinant viruses. Researchers utilize 293FT cells to produce viral vectors for gene delivery, to study gene function and regulation, and to develop gene therapies for various diseases. Their role in the production of viral vectors makes 293FT cells a cornerstone in the fields of gene therapy, functional genomics, and molecular cloning, facilitating the advancement of research and therapeutic development.