HEL Cells
| Unit |
5 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Leukemia cell lines |
| Organism |
Human |
| Donor History |
Erythroleukemia |
| Growth Properties |
Suspension |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
HEL cells are a human erythroleukemia cell line that was established from the peripheral blood of a 30-year-old man with erythroleukemia in relapse after treatment for Hodgkin lymphoma in 1980.
HEL cells are capable of spontaneous and induced globin synthesis, producing mainly G gamma and A gamma chains. These cells also express embryonic chains (epsilon, zeta) and alpha chains in minimal amounts, while beta chains are undetectable.
HEL cells are round, large to occasionally giant polynucleated, single cells in suspension, with a few cells adherent. The expression of mutated JAK2 has been confirmed in these cells by RT-PCR and sequencing. HEL cells express several cell surface markers, including CD3-, CD13+, CD14-, CD19-, CD33+, CD41a+, CD71+, and CD235a+.
According to research, hydroxyurea, a medicine routinely used to treat a variety of cancers, including erythroleukemia, may also regulate the death of HEL cells.
HEL cell apoptosis produced by hydroxyurea may be connected to HEL cell terminal differentiation. Additionally, earlier research has shown that hydroxyurea may be crucial in controlling HEL cell proliferation and differentiation.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

