HeLa EB3 GFP KIRC14 Cells

HeLa EB3-GFP Kirc 14 is a genetically modified derivative of the human HeLa cervical carcinoma cell line, engineered to stably express a GFP-tagged EB3 (end-binding protein 3) fusion construct, with “Kirc 14” denoting a specific sub-clone (clone 14) selected from the transfected pool. EB3 is a member of the end-binding protein family that tracks growing microtubule plus-ends and serves as a reporter for dynamic microtubule behaviour in living cells. The GFP-EB3 fusion protein produces characteristic comet-like fluorescent signals at polymerizing microtubule tips, enabling live-cell imaging of microtubule dynamics, intracellular transport, and cytoskeletal organisation in real time.
HeLa EB3-GFP Kirc 14 is used in cell biology research for live-cell imaging of microtubule plus-end dynamics, studies of mitotic spindle assembly and kinetochore-microtubule interactions, investigations of intracellular cargo transport, and evaluation of compounds that affect microtubule polymerization dynamics. The stable sub-clonal GFP-EB3 expression provides consistent fluorescence intensity and pattern across passages, making clone 14 suitable for quantitative imaging assays and high-throughput screening of microtubule-targeting agents.