HK EGFP-alpha-tubulin/H2B-mCherry Cells

The HK EGFP-alpha-tubulin/H2B-mCherry HeLa Kyoto cell line is a meticulously engineered model designed for detailed visualization of cellular processes. This clonal line has been stably transfected to express two fluorescent protein fusions that enable real-time imaging of both chromatin and the microtubular network. The red fluorescent protein mCherry is fused to the core histone protein H2B, creating H2B-mCherry. This fusion protein is expressed from the pH2B-mCherry-IRES-neo3 plasmid and serves as a chromatin marker, highlighting the nuclear DNA in live-cell imaging and facilitating studies on chromatin dynamics and nuclear architecture.
Additionally, this cell line expresses monomeric enhanced GFP (Green Fluorescent Protein) fused to α-tubulin, introduced via the pmEGFP-α-tubulin-IRES-puro2b plasmid. The GFP-α-tubulin fusion provides a vivid green fluorescence that outlines the microtubule structures within the cell. This feature is crucial for studying microtubule organization, dynamics, and their role in cell division and intracellular transport. The stable integration of these constructs allows for continuous, long-term observation of these cellular components without the need for repeated transfection, thus reducing variability and enhancing the reliability of experimental results. Drug resistance selection following transfection ensures the stability and uniformity of expression among the cells in this line.