HK EGFP-LaminA/H2B-mCherry Cells

The HK EGFP-LaminA/H2B-mCherry cell line is a genetically engineered Hela Kyoto derived cell model developed to facilitate advanced studies in nuclear dynamics and chromatin organization within living cells. This cell line expresses two fusion proteins: EGFP (enhanced green fluorescent protein) fused with Lamin A, and mCherry (a red fluorescent protein) fused with Histone H2B. The EGFP-Lamin A fusion highlights the nuclear envelope and allows for the visualization of nuclear architecture changes during cell cycle progression or under various experimental conditions. Meanwhile, the H2B-mCherry fusion protein binds to DNA and provides a vivid red fluorescence that marks chromatin, enabling real-time observation of chromosomal processes during mitosis and interphase.

These cells are invaluable for real-time imaging applications, including studies on nuclear integrity, DNA replication, and cellular aging, as well as research into diseases where nuclear architecture is disrupted, such as cancer and laminopathies. The dual-color fluorescence feature of this cell line allows for simultaneous visualization of both the nuclear envelope and chromatin, facilitating a comprehensive understanding of nuclear-cytoplasmic interactions and the spatiotemporal organization of chromatin. Such capabilities make it a critical tool for molecular biology research and cellular biophysics, providing insights into the mechanics of gene expression regulation, nuclear organization, and the cell cycle.