HK EGFP-LaminB1/H2B-mCherry Cells

The HK EGFP-LaminB1/H2B-mCherry cell line is an Hela Kyoto derived in vitro model designed for real-time visualization of chromatin dynamics and nuclear architecture in living cells. This cell line expresses two fluorescent protein fusions: EGFP (enhanced green fluorescent protein) fused with Lamin B1, and mCherry (a red fluorescent protein) fused with histone H2B. The fusion of EGFP with Lamin B1 allows for the observation of the nuclear envelope and nuclear lamina, structures critical for maintaining the integrity and functionality of the nucleus. Lamin proteins are type V intermediate filament proteins that form a meshwork underlying the inner nuclear membrane, playing key roles in nuclear stability, chromatin organization, and gene regulation.

On the other hand, the mCherry-tagged histone H2B enables the visualization of chromatin within the nucleus. Histones are fundamental components of the nucleosome, involved in the organization of DNA into chromatin, making them crucial for DNA replication, repair, and transcription. The mCherry tag on H2B provides a vivid red fluorescence that contrasts with the green fluorescence of EGFP, allowing for simultaneous dual-imaging of the nuclear structure and chromatin in live-cell experiments. This cell line is commonly used in studies focusing on nuclear mechanics, mitosis, and genome stability, providing a dynamic view of cellular processes that are otherwise difficult to observe in real time.