HK-ZFN-AURKB-mEGFP/ZFN-INCENP-mCherry Cells

Unit

3 x 10^6 cells/ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Reporter-labeled cells

Organism

Human

Donor History

Adenocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HK-ZFN-AURKB-mEGFP-ZFN-INCENP-mCherry-Cells/300270

Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The HK-ZFN-AURKB-mEGFP/ZFN-INCENP-mCherry cell line, derived from HeLa Kyoto cells, is a specialized model used in cellular biology research. It has been genetically engineered to express Aurora B kinase (AURKB) tagged with monomeric enhanced green fluorescent protein (mEGFP) and Inner Centromere Protein (INCENP) tagged with mCherry. These modifications enable researchers to track the dynamics and interactions of these proteins during cell division. Aurora B kinase is essential for chromosome segregation and cytokinesis, while INCENP is a critical component of the Chromosomal Passenger Complex (CPC), coordinating mitotic progression.

This dual fluorescent tagging provides a powerful tool for live-cell imaging, allowing detailed study of protein distribution during the cell cycle. The HK-ZFN-AURKB-mEGFP/ZFN-INCENP-mCherry cell line is valuable for researching mitotic regulation, chromosomal stability, and the mitotic checkpoint. The precision of zinc finger nucleases (ZFNs) used for genetic modifications ensures the accuracy of this model, making it ideal for high-fidelity studies in cancer biology and therapeutic development.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.