Genetika člověka
Mikrobiologické aplikace
NGS
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Pipetovací špičky
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Pipetovací roboty
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Jednokanálové
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Biochemie
Imunoanalýza
HlPrx Stable BHK-21 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Golden Hamster (M. auratus) |
| Donor History | |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
HlPrx Stable BHK-21 Cell Line are developed to stably express Haemaphysalis longicornis derived 1-Cys peroxiredoxin (HlPrx) through transfection with pmirGLO vector with the HlActin promoter. Monoclonal selection was conducted with the presence of G418. H2O2-scavenging enzymes (Prxs( are important in ticks oxidative stress coping mechanism and have been characterized in tick biology and in relationship to tick pathogens. Thus this cell line may be used to study Prx and its role in tick-host-pathogen interactions.
