HNO41 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Head-Neck cancer cell lines

Organism

Human

Donor History

Head and neck squamous cell carcinoma (HNSCC)

Growth Properties

Monolayer

,

adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HNO41-Cells/300126

SKU: 300126 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The HNO41 cell line is derived from a hypopharyngeal squamous cell carcinoma, a type of head and neck squamous cell carcinoma (HNSCC). This cell line has been characterized by several chromosomal aberrations, including DNA copy number gains in chromosomal regions such as 3q23-qter, 5p, 7p, 7q21-q22, 8q22.2-qter, 9q22-qter, and 11q13. These regions are known to harbor oncogenes that contribute to tumor progression, making HNO41 a valuable model for studying the molecular mechanisms underlying hypopharyngeal cancer.

In addition to its genetic profile, HNO41 has been analyzed for its expression of angiogenic growth factors, which are critical in tumor development and metastasis. The cell line exhibits strong expression of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF), among others. These factors are involved in promoting angiogenesis, the formation of new blood vessels, which is a key process in tumor growth and metastasis. The presence of these factors in HNO41 further supports its utility in research focused on understanding tumor angiogenesis and in evaluating anti-angiogenic therapies for HNSCC.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.