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hPIM1 CRISPR Knock Out SACC-83 Cell Line-T3-9
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Salivary Gland |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,Salivary gland adenoid cystic carcinoma |
| Growth Properties | |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.6 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/hpim1-crispr-knock-out-sacc-83-cell-line-t3-9.html |
This cell line features a CRISPR-Cas9 mediated knockout of the human Pim-1 Proto-Oncogene (PIM1) which encodes for the Serine/Threonine Kinase of the same name, in the SACC-83 cell line. The cell line is suitable for 3D cell culture and drug discovery related research.
