HROC40 Cells

Culture medium: DMEM:Ham's F12 (1:1)

w: 3.1 g/L Glucose

w: 2.5 mM L-Glutamine

w: 15 mM HEPES

w: 0.5 mM Sodium pyruvate

w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 5×10⁴ cells/cm² after thawing

3×10⁴ cells/cm² once the cells are proliferating vigorously. Freeze medium: As a cryopreservation medium

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

or CM-1 (Cytion catalog number 800100)

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

5% CO2

humidified atmosphere.