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Hs2st1 Stable Knockout Heparan Sulfate Cell Line (Hs2st1-/-)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Lung |
| Organism |
Mouse (M. musculus) |
| Donor History |
Adult C57BL6 |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. PriGrow III (TM003) + 10% FBS + 30µg/mL Endothelial Cell Growth Supplement (TM130) + 50µg/mL Heparin + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Hs2st1-Stable-Knockout-Heparan-Sulfate-Cell-Line-Hs2st1-.html |
This cell line (Cat. T3378) featues a stable knockout of heparan sulfate (HS) 2-O-sulfotransferease (Hs2st1) knockout using Cre-Lox recombinase. The control cell line for this catalog is Cat. T3377 – Hs2st1 Wild-Type Heparan Sulfate Stable Cell Line (Hs2st1f/f). Target gene deletion was confirmed using genomic DNA genotyping. This cell line is part of a comprehensive heparan sulfate (HS)-mutant mouse lung endothelial cell (MLEC) library. These cell lines are suitable for investigations examining the roles and structures of HS within the cellular context. Complete list of mutant and wild-type heparan sulfate mouse lung endothelial cell lines from this panel:Cat. T3373 – Ndst1 Wild-Type Heparan Sulfate Stable Cell Line (Ndst1f/f)Cat. T3374 – Ndst1 Stable Knockout Heparan Sulfate Cell Line (Ndst1-/-)Cat. T3375 – Ndst1f/f ;Ndst2-/- Heparan Sulfate Stable Cell Line (Ndst1f/f ;Ndst2-/-)Cat. T3376 – Glce Stable Knockout Heparan Sulfate Cell Line (Glce-/-)Cat. T3377 – Hs2st1 Wild-Type Heparan Sulfate Stable Cell Line (Hs2st1f/f)Cat. T3378 – Hs2st1 Stable Knockout Heparan Sulfate Cell Line (Hs2st1-/-)Cat. T3379 – Hs6st1 Wild-Type Heparan Sulfate Stable Cell Line (Hs6st1f/f)Cat. T3380 – Hs6st1 Stable Knockout Heparan Sulfate Cell Line (Hs6st1-/-)Cat. T3381 – Hs6st1f/f;Hs6st2-/- Heparan Sulfate Stable Cell Line (Hs6st1f/f;Hs6st2-/-)Cat. T3382 – Hs6st1;Hs6st2 Stable Knockout Heparan Sulfate Cell Line (Hs6st1-/-;Hs6st2-/-)Cat. T3383 – Hs3st1 Stable Knockout Heparan Sulfate Cell Line (Hs3st1-/-)Cat. T3384 – Hs3st4 Stable Knockout Heparan Sulfate Cell Line (Hs3st4-/-)Cat. T3385 – Hs3st1;Hsst4 Stable Knockout Heparan Sulfate Cell Line (Hs3st1-/-;Hsst4-/-)Cat. T3386 – Sulf1/Sulf2 Wild-Type Heparan Sulfate Stable Cell Line (Sulf1f/f;Sulf2f/f)Cat. T3387 – Sulf1/Sulf2 Stable Knockout Heparan Sulfate Cell Line (Sulf1-/-;Sulf2-/-)Cat. T3490 – Ndst1/Ndst2 Stable Knockout Heparan Sulfate Cell Line (Ndst1-/- ;Ndst2-/-)Cat. T3491 – Ext1 Wild-Type Heparan Sulfate Stable Cell Line (Ext1f/f)Cat. T3492 – Ext1 Stable Knockout Heparan Sulfate Cell Line (Ext1-/-)
