Human Hematopoietic‐Myeloid Leukemia Cell Line (AML14.3D10)

PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. PriGrow II (TM002) + 8% FBS + 2mM L-glutamine (G275) + 1mM Sodium Pyruvate (TM057) + 50uM Beta- mercaptoethanol + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. Note: Cells are difficult to recover from freeze-thaw conditions and typically show 20-30% viability post-thaw; allow cells to recover in the first 72 hours through frequent media changes. It is absolutely imperative to perform a cell viabilty count each time cells are passaged  to keep them between 3 x 105 – 1 x 106 maximum/ml. Do not allow the cells to achieve a density greater than 8 X 105 viable cells/ml.; this will lead to terminal differentiation and cell death. Addition of 100 pg/ml GM-CSF to the culture media during the first week post-thaw will allow for more rapid recovery and improved cell viability.