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Imunoanalýza
Human PIK3R1 CRISPR Knockout TeloHAEC Cell Line – AP209
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Heart |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,23 ,Normal ,Aorta |
| Growth Properties |
Adherent ,endothelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium (TM3499) + 1.6% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.3ug/mL Puromycin (G264) and 2ug/ml blasticydin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Human-PIK3R1-CRISPR-Knockout-TeloHAEC-Cell-Line-209-T3499.html |
This cell line has been genetically modified using CRISPR/Cas9 technology to knock out the PIK3R1 gene, which encodes the regulatory subunit of phosphoinositide 3-kinase (PI3K). The absence of PIK3R1 disrupts PI3K signaling, providing a valuable model for studying the role of PI3K in endothelial function, vascular biology, and related signaling pathways. Applications of this stable cell line include research in cardiovascular diseases, angiogenesis, and cancer biology, offering insights into the development of targeted therapies and the exploration of PI3K-related cellular mechanisms.
