Human Primary Alveolar Cells – Type 1
| Unit |
5×10⁵ cells / 1.0 ml |
|---|---|
| Cell Type |
Primary Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History |
Normal tissue |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. PriGrow X Series Medium (TM4003) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.Note: change medium every day/other day to remove nonadherent cells and replenish nutrients. Once cells are greater than 70% confluent ,change media daily. Cells can be expanded for 3-6 passages using a 1:2 split ratio. |
| Markers |
CK-18 ,CK-19 |
| Full Information |
https://www.abmgood.com/human-normal-alveolar-cells-type-1.html |
Type I alveolar epithelial cells extend across several air sacs and cover more than 95% of the alveolar surface area. They are specialized to serve as very thin (often only 25 nm in width) gas-permeable components of the blood-air barrier. The Type I Alveolar cells are the target of many insults, including ventilator-induced lung injury. These cells are useful in investigating the function and pathology of the respiratory system.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
