Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Human Primary Cardiomyocytes
| Unit |
5×10⁵ cells / 1.0 ml |
|---|---|
| Cell Type |
Primary Cells |
| Tissue Type |
Heart |
| Organism |
Human (H. sapiens) |
| Donor History |
Normal tissue |
| Growth Properties |
Adherent ,multipolar |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium (TM4037) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.Cells can be expanded for up to 15 population doublings. Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. |
| Markers |
Sarcomeric alpha-actinin ,slow muscle myosin |
| Full Information |
Human Primary Cardiomyocytes are isolated from human heart ventricles. Their specialized high-oxygen-content with a large number of mitochondria contributes to the major role of cardiac muscles in the heart’s rhythmic pumping. Cardiomyocytes are regulated by a complex network of signals. Cardiomyocytes hypertrophy and apoptosis have been associated with the loss of contractile function during heart failure. They are ideal models for study of cytokines and cellular signaling mechanisms that lead to myocyte death, as well as for research on mechanical strain and cell-cell interaction.
