Genetika člověka
Mikrobiologické aplikace
NGS
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PCR, qPCR, RT
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Biochemie
Imunoanalýza
Immortalized Human Corneal Fibroblasts (IHCF)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Eye |
| Organism |
Human (H. sapiens) |
| Donor History |
Not disclosed |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions | |
| Immortalization Method |
Serial passaging and transduction with retroviruses carrying human papillomavirus type 16 E6/E7 gene |
| Full Information |
https://www.abmgood.com/immortalized-human-corneal-fibroblasts-ihcf.html |
Tissue repair and wound healing in the cornea is accomplished mainly by the corneal fibroblasts, and these cells are responsible for pathological process in the degenerative corneal disorders such as keratoconus. Established using the HPV16 E6/E7 genes, The Immortalized Human Corneal Fibroblasts (IHCF) not only retains its authentic cell biomarkers (CD36, ECM protein collagen I and S100A4), it also displays fairly disomic cytogenetic status at chromosomes 1, 8, 10 and 18, which is an indication of normal diploid chromosomal status.Together with the Immortalized Human Corneal Keratinocytes (T0579) and the Immortalized Human Corneal Endothelial Cells (T0577) isolated from the same donor and immortalized using the same platform, this uniform model system presents a valuable tool for studies involving cornea homeostasis and ophthalmologic diseases. RT-PCR was used to evaluate the transgene expression of HPV16 E6/E7.
