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Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Human (H. sapiens) |
| Donor History |
8-week-old fetal human ventral mesencephalon |
| Growth Properties |
Adherent ,flat dendritic processes |
| Growth Conditions | |
| Immortalization Method |
Conditional immortalization by tetracyclin-controlled transduction with retrovirus carrying v-myc genes |
| Full Information |
https://www.abmgood.com/immortalized-human-dopaminergic-neuronal-precursor-cells-luhmes.html |
The Immortalized Human Dopaminergic Neuronal Precursor Cells, also known as the Lund Human Mesencephalic (LUHMES) cell line, is a subclone of the tetracycline-controlled, v-myc-overexpressing human mesencephalic-derived cell line MESC2.10. This cell line is unique in that it can be differentiated to acquire a dopaminergic neuron-like phenotype under appropriate growth conditions. LUHMES expresses functional dopamine transporter (DAT), vesicular monoamine transporter (VMAT-2), tyrosine hydroxylase (TH) and the neuronal form of β-III tubulin after differentiation. In addition to retaining dopaminergic neuronal-specific markers, LUHMES also exhibit electrophysiological properties, thus making this cell line a valuable neuronal model for neurodevelopmental studies, disease modelling and neuropharmacology. May take 4 to 5 days for cells to recover in culture.
