Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
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PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
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Imunologické analyzátory
Veterinární imunologie
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Intolerance u zvířat
Biochemie
Imunoanalýza
Immortalized Human Mammary Fibroblasts (HMF3A)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Mammary |
| Organism |
Human (H. sapiens) |
| Donor History |
Not disclosed |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions | |
| Immortalization Method |
Combined transduction with retrovirus carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT) |
| Full Information |
https://www.abmgood.com/immortalized-human-mammary-fibroblasts-hmf3a.html |
Immortalized Human Mammary Fibroblasts (HMF3A) were generated through the combined transduction of mammary fibroblasts with a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT). The HMF3A cells grow best at a temperature of 33.5⁰C and undergo complete arrest at 39.5⁰C. These cells are temperature sensitive unlike the Immortalized Human Mammary Fibroblasts (HMF3S) (Cat. No. T0154). These conditionally immortalized cells are suitable for cell engineering and are theoretically capable of generating immortal diploid human lines.Telomerase-detection assay was used to detect telomerase activity.
