Immortalized Human Mammary Fibroblasts (HMF3S)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Mammary |
| Organism |
Human (H. sapiens) |
| Donor History |
Not disclosed |
| Growth Properties |
Adherent ,spindle-shaped |
| Growth Conditions | |
| Immortalization Method |
Combined transduction with retrovirus carrying a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT) |
| Full Information |
https://www.abmgood.com/immortalized-human-mammary-fibroblasts-hmf3s.html |
Immortalized Human Mammary Fibroblasts (HMF3S) were generated through the combined transduction of mammary fibroblasts with a temperature sensitive mutant (U19tsA58) of the SV40 Large T Antigen and a catalytic subunit of human telomerase (hTERT). The HMF3S cells grow best at a temperature of 37⁰C and are not temperature sensitive compared to the Immortalized Human Mammary Fibroblasts (HMF3A) (Cat. No. T0153).Telomerase-detection assay was used to detect telomerase activity.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
